Reproduction+(heredity)

Reproduction  Brooke ; Alyssa ; Matt ; Dylan

__Hypothesis: __

If less light is able to get through the test tube over time, the substance has reproduced and therefore is living.

__Material: __

1. Two test tubes with screw caps 2. Test tube rack 3. Spectrophotometer 4. Pipette and pump 5. Glass marking pencil 6. Known and unknown substance

__Procedure: __

1. Label the two test tubes-  Test tube 1: negative control  Test tube 2: unknown substance A  Test tube 3: unknown substance B

<span style="font-family: Arial,Helvetica,sans-serif;">2. Mix each substance with ¾ of broth- <span style="font-family: Arial,Helvetica,sans-serif;"> Test tube 1: only broth <span style="font-family: Arial,Helvetica,sans-serif;"> Test tube 2: broth and 1/6 unknown substance A <span style="font-family: Arial,Helvetica,sans-serif;"> Test tube 3: broth and 1/6 unknown substance B

<span style="font-family: Arial,Helvetica,sans-serif;">3. Cover the tube with the cap

<span style="font-family: Arial,Helvetica,sans-serif;">4. Take the spectrophotometer readings from all tubes

<span style="font-family: Arial,Helvetica,sans-serif;">5. Repeat spectrophotometer readings for four days

<span style="font-family: Arial,Helvetica,sans-serif;">6. Create a table and record your data

<span style="font-family: Arial,Helvetica,sans-serif;">7. Graph data recorded in the table

__<span style="font-family: Arial,Helvetica,sans-serif;">RECORDING: __

<span style="font-family: Arial,Helvetica,sans-serif;">DAY 1: <span style="font-family: Arial,Helvetica,sans-serif;"> Test tube 1: 100.4 <span style="font-family: Arial,Helvetica,sans-serif;"> Test tube 2: 15.6 <span style="font-family: Arial,Helvetica,sans-serif;"> Test tube 3: 0.2

<span style="font-family: Arial,Helvetica,sans-serif;">DAY 2: <span style="font-family: Arial,Helvetica,sans-serif;">Test tube 1: 107.8 <span style="font-family: Arial,Helvetica,sans-serif;"> Test tube 2: 10.4 <span style="font-family: Arial,Helvetica,sans-serif;"> Test tube 3: 0.2

DAY 4: Test tube 1: 41.6 Test tube 2: 3.4 Test tube 3: 0.6

-You never tested Test tube C